Analysis of Aristolochic Acids in Aristolochia consimilis and its derived commercial products
نویسندگان
چکیده
ii Chapter I General Introduction 1 Chapter II Literature review 4 2.1 Aristolochia 5 2.1.1 Aristolochia consimilis 5 2.2 Pharmacology 6 2.3 Aristolochic Acid 8 2.3.1 Toxicity 9 2.4 Methods for analyzing aristolochic acids 11 2.4.1 Extraction of aristolochic acids 11 2.4.2 Thin layer chromatography (TLC) 12 2.4.3 High performance liquid chromatography (HPLC) 12 2.5 Objective 14 2.6 Approach 14 Chapter III Materials and Methods 15 3.1 Plant material 15 3.2 Chemicals 15 3.3 Sample preparation and extraction 16 3.4 TLC analysis 17 3.5 HPLC equipment and chromatographic conditions 17 3.6 Standard solution and calibration curve 18 3.7 Method validation 18 3.7.1 Linearity, limit of detection and limit of quantification 19 3.7.1 Precision and accuracy 19 3.8 Sample preparation and GC-MS analysis 19 3.9 Solid phase extraction (SPE) and 1 H NMR spectroscopy 19 3.10 Statistical analysis 19 Chapter IV Results and Discussion 22 4.1 Ethnopharmacological study 22 4.2 Thin layer chromatography 25 4.3 Method development 26 4.4 Extraction of Aristolochic acids 28 4.4.1 Effect of solvent type and extraction time 28 4.4.2 Extraction efficiency 30
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